불화나트륨 투여에 의한 백서경조직 미세경도 및 골강도 변화
Effect of Sodium Fluoride on surface Microhardness and Strength in the Rat Bones
소속 상세정보
최성진/Choi, Sung Jin
송근배/Song, Keun Bae
KMID : 0355419970210010117
Abstract
The objectives of this study were to investigate the changes in rat bones after short periods of administration of sodium fluoride.
Twenty-four Sprague-Dawley strain female rats were divided into four groups, which took distilled water containing 0, 1, 10 and 100 ppm fluoride, respectively for 3 weeks. The animals were killed, weighted and both sides of femur, tibia, mandible and maxilla were dissected. The slabs of each bone were embedded in unsaturated polyester, polished with # 1,200 silicon carbide paper and aluminosilicate powder, and were tested for surface microhardness using the Vickers microhardness tester fitted with an diamond indenter. The other"sides of femur and tibia were marked in the exact center and placed across a 1cm bridge, in such a manner that positioning was uniform from bone to bone, and loaded perpendicular to its long axis. The load was applied at the midpoint of the bone at the rate of 1mm per minute until it fractured, using an Instron testing instrument.
Data analysis were conducted using the SPSS/PC+ package. Differences in each group were compared by oneway ANOVA and the Duncan´s multiple range test. Corresponding p-value were considered significant at values < 0.05.
The obtained results were as follows
There were no significant differences in weight change of the rats except the 10
ppm fluoride administration group.
Surface microhardness of femur, mandible and maxilla were increased with high fluoride concentration in the drinking water.
The stress at fracture of the femur and tibia were similar in control animals and those 1 and 10 ppm of fluoride. However, bones from animals receiving 100 ppm of fluoride withstood less stress at fracture than animals of control, 1 and 10 ppm groups.
All of the findings presented support the conclusion that fluoride had the expected biological effect on bone. Within the range of doses used and under the specific conditions of this experiment, there was an increase of surface microhardness of bone with the administration of fluoride. In view of the three point bending tests of femur and tibia, the fluorotic bone was more brittle than the controls
키워드
불화나트륨;백서경조직;미세경도;골강도
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